RAFT of sulfobetaine for modifying poly(glycidyl methacrylate) microspheres to reduce nonspecific protein adsorption

摘要: The minimization of nonspecific protein adsorption is a crucial step in the development bioseparation processes, immunoassays, and affinity diagnostics. Among numerous biomaterials, polyzwitterions are known to effectively suppress cell adhesion. This article describes formation monodisperse polymer microspheres coated with polysulfobetaine aim limit bovine serum albumin (BSA) as model protein. In this process, 2-μm poly(glycidyl methacrylate) (PGMA) were prepared by dispersion polymerization. To render hydrophilic biocompatible, [3-(methacryloylamino)propyl]dimethyl(3-sulfopropyl)ammonium hydroxide (MPDSAH) was grafted from surface reversible addition-fragmentation chain transfer (RAFT) Elemental analysis modified revealed up 20 wt % poly{[3-(methacryloylamino)propyl]dimethyl(3-sulfopropyl)ammonimum hydroxide} (PMPDSAH). characterized terms particle size, morphology, zeta potential. amount BSA nonspecifically adsorbed on PMPDSAH-modified decreased half that captured unmodified PGMA microspheres. © 2015 Wiley Periodicals, Inc. J. Polym. Sci., Part A: Chem. 2015, 53, 2273–2284