Unravelling the Genetic Diversity among Cassava Bemisia tabaci Whiteflies Using NextRAD Sequencing.

作者: Everlyne N Wosula , Wenbo Chen , Zhangjun Fei , James P Legg

DOI: 10.1093/GBE/EVX219

关键词:

摘要: Bemisia tabaci threatens production of cassava in Africa through vectoring viruses that cause mosaic disease (CMD) and brown streak (CBSD). B. sampled from eight countries were genotyped using NextRAD sequencing, their phylogeny population genetics investigated the resultant single nucleotide polymorphism (SNP) markers. SNP marker data short sequences mitochondrial DNA cytochrome oxidase I (mtCOI) obtained same insect compared. Eight genetically distinct groups identified based on mtCOI, whereas phylogenetic analysis SNPs six major groups, which further confirmed by PCA multidimensional analyses. STRUCTURE four ancestral populations have contributed alleles to SNP-based groups. Significant gene flows detected between several Evidence flow was strongest for occurring central Africa. Comparison mtCOI identities insects provided a strong indication hybrid are emerging parts recently affected severe CMD pandemic. This study reveals is not an effective at distinguishing cassava-colonizing haplogroups, more robust multilocus markers should be developed. could lead emergence haplogroups might alter dynamics virus spread severity Continuous monitoring genetic compositions whitefly essential component efforts combat

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