Characterization of the V0 domain of the coated vesicle (H+)-ATPase.
作者： J Zhang , M Myers , M Forgac
DOI: 10.1016/S0021-9258(19)50159-4
关键词:
摘要: The coated vesicle (H+)-ATPase is composed of two domains, a peripheral V1 domain containing the 73 (A subunit)-, 58 (B 40-, 34-, and 33-kDa subunits an integral V0 100-, 38-, 19-, 17 (c subunit)-kDa (Adachi, I., Puopolo, K., Marquez-Sterling, N., Arai, H., Forgac, M. (1990) J. Biol. Chem. 265, 967-973). In present manuscript we characterize with respect to its structural activity properties. Glycerol density gradient separation solubilized membrane proteins reveals presence excess domains which migrate molecular weight 250,000 contain polypeptides in same stoichiometry as intact V1V0 complex. Like c subunit V1V0, free labeled by [14C]N,N'-dicyclohexylcarbodiimide (DCCD) extracted chloroform:methanol. addition, monoclonal antibody specific for 100-kDa recognizes V0. Tryptic cleavage complex gives pattern fragments 100- 38-kDa complex, but increase sensitivity, suggesting greater exposure these Proton conduction was measured reconstituted vesicles native stripped V1. No DCCD-inhibitable proton observed either preparation, that unlike corresponding F0 F1F0, not open channel.
sci-hub.st 参考文章(31)
Carl W. Anderson, J.William Straus, Bernard S. Dudock, Preparation of a cell-free protein-synthesizing system from wheat germ. Methods in Enzymology. ,vol. 101, pp. 635- 644 ,(1983) , 10.1016/0076-6879(83)01044-7
H.W. Heldt, U.I. Flügge, [56] Transport of metabolites across the chloroplast envelope Methods in Enzymology. ,vol. 125, pp. 705- 716 ,(1986) , 10.1016/S0076-6879(86)25058-2
K Ko, O Bornemisza, L Kourtz, Z.W. Ko, W.C. Plaxton, A.R. Cashmore, Isolation and characterization of a cDNA clone encoding a cognate 70-kDa heat shock protein of the chloroplast envelope. Journal of Biological Chemistry. ,vol. 267, pp. 2986- 2993 ,(1992) , 10.1016/S0021-9258(19)50684-6
D J Schnell, G Blobel, D Pain, Signal peptide analogs derived from two chloroplast precursors interact with the signal recognition system of the chloroplast envelope. Journal of Biological Chemistry. ,vol. 266, pp. 3335- 3342 ,(1991) , 10.1016/S0021-9258(18)49993-0
K J Clemetson, D Bienz, Human Platelet Glycoprotein Ia: One component is only expressed on the surface of activated platelets and may be a granule constituent Journal of Biological Chemistry. ,vol. 264, pp. 507- 514 ,(1989) , 10.1016/S0021-9258(17)31287-5
S.E. Perry, W.E. Buvinger, J. Bennett, K. Keegstra, Synthetic analogues of a transit peptide inhibit binding or translocation of chloroplastic precursor proteins. Journal of Biological Chemistry. ,vol. 266, pp. 11882- 11889 ,(1991) , 10.1016/S0021-9258(18)99040-X
M. Ikeuchi, Y. Inoue, Specific 125
I labeling of D1 (herbicide-binding protein) FEBS Letters. ,vol. 210, pp. 71- 76 ,(1987) , 10.1016/0014-5793(87)81300-5
PH O'Farrell, High resolution two-dimensional electrophoresis of proteins. Journal of Biological Chemistry. ,vol. 250, pp. 4007- 4021 ,(1975) , 10.1016/S0021-9258(19)41496-8
L. P. Vernon, Spectrophotometric Determination of Chlorophylls and Pheophytins in Plant Extracts Analytical Chemistry. ,vol. 32, pp. 1144- 1150 ,(1960) , 10.1021/AC60165A029
L. Mendiola-Morgenthaler, S. Leu, A. Boschetti, Isolation of biochemically active chloroplasts from chlamydomonas Plant Science. ,vol. 38, pp. 33- 39 ,(1985) , 10.1016/0168-9452(85)90076-7