The antiproliferative function of violacein-like purple violet pigment (PVP) from an Antarctic Janthinobacterium sp. Ant5-2 in UV-induced 2237 fibrosarcoma.

摘要: Background  In this study, we have investigated the chemotherapeutic potential of a purple violet pigment (PVP), which was isolated from previously undescribed Antarctic Janthinobacterium sp. (Ant5-2), against murine UV-induced 2237 fibrosarcoma and B16F10 melanoma cells. Methods  The 2237, B16F10, C50, NIH3T3 cells were treated with PVP at different doses for times, their proliferation viability detected by 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) assay. Cell cycle arrest induced in assessed flow cytometry expression analysis cell regulatory proteins done Western blot. Apoptosis observed annexin-V/propidium iodide double staining assay fluorescence microscopy. To further determine molecular mechanism apoptosis PVP, changes Bcl-2, Bax cytochrome c loss mitochondrial membrane 2237 cells JC-1 dye following cytometry. Caspase-3, Caspase-9 PARP cleavage analyzed blot Caspase-3 -9 activities measured colorimetric assays. Results In vitro treatment resulted decreased (13–79%) time (24–72 h) dose (0.1–1 μm)-dependent manner. PVP-induced growth inhibition associated both G0/G1 G2/M phase accompanied decrease cyclin dependent kinases (Cdks) simultaneous increase kinase inhibitors (Cdki) – Cip1/p21 Kip1/p27. Further, significant an increased pro-apoptotic protein Bax, anti-apoptotic disruption potential, release, activation caspase-3, caspase-9 poly-ADP-ribose-polymerase (PARP) cleavage. Conclusions  We describe anti-cancer first bacterium suggest that could be used as potent agent nonmelanoma skin cancers.