MicroRNAs of Toxocara canis and their predicted functional roles

摘要: Toxocara canis is the causative agent of toxocariasis humans and other animals. This parasitic nematode (roundworm) has a complex life cycle, in which substantial developmental changes switches occur. As small non-coding RNAs (sRNAs) are key regulators gene expression wide range organisms, we explored these T. to provide basis for future studies its biology as well host interactions disease at molecular level. We conducted high-throughput RNA sequencing bioinformatic analyses define sRNAs individual male female adults canis. Apart from snRNA snoRNA, 560 619 microRNAs (miRNAs), including 5 2 novel miRNAs, were identified worms, respectively, without piRNAs being detected either sex. An analysis transcriptional profiles showed that, 564 miRNAs predicted differentially transcribed between individuals canis, 218 exclusively 277 worms. Functional enrichment that both mainly involved regulating embryonic morphogenesis, hemidesmosome assembly genetic information processing. The sexes be associated with sex determination, morphogenesis larval development. roles based on ontology (GO) KEGG pathway annotations. Tc-miR-2305 Tc-miR-6090 proposed have reproduction, embryo development development, Tc-let-7-5p, Tc-miR-34 Tc-miR-100 appear host-parasite interactions. Together published previous studies, some (such Tc-miR-2861, Tc-miR-2881 Tc-miR-5126) represent drug targets and/or resistance. first exploration could fundamental investigations parasite, parasite-host applied areas, such diagnosis infection/disease, target discovery resistance detection.