Porphyromonas gingivalis Uses Specific Domain Rearrangements and Allelic Exchange to Generate Diversity in Surface Virulence Factors

摘要: Porphyromonas gingivalis is a keystone pathogen of chronic periodontitis. The virulence P. reported to be strain related and there are currently number typing schemes based on variation in capsular polysaccharide, the major minor fimbriae adhesin domains Lys-gingipain (Kgp), amongst other surface proteins. can exchange chromosomal DNA between strains by natural competence conjugation. aim this study was determine genetic variability sourced from international locations over 25-year period if factors has phylogenetic basis. Whole genome sequencing performed 13 comparison made 10 previously sequenced strains. A single nucleotide polymorphism-based analysis demonstrated shallow tri-lobed phylogeny. There high level reticulation network, demonstrating extensive horizontal gene transfer Two highly conserved variants catalytic domain factor Kgp proteinase (KgpcatI KgpcatII) were found. three fourth C-terminal cleaved domain. Specific cell proteins FimA, FimCDE, MfaI, RagAB, Tpr, PrtT also identified. occurrence all these formed mosaic that not SNP-based In conclusion uses rearrangements generate diversity specific factors.