The little brown bat nuclear genome contains an entire mitochondrial genome: Real or artifact?

摘要: Abstract Nuclear mitochondrial DNA sequences (NUMTs) have been documented in almost all eukaryotic genomes studied. Recently, with the number of sequenced increasing, extremely large NUMTs, even a nearly entire genome, reported some plants and animals. However, few such studies provided strong experimental evidences for these important discoveries. In this study using computer-based search method an genome (NUMT-1) was found nuclear bat species ( Myotis lucifugus ). This super-large NUMT shared same scaffold 754 bp genomic sequence second (NUMT-2, 3292 bp). If NUMT-1 real, it will be largest animals finding provide valuable insights into mode generation NUMTs genome. Unfortunately, although initial sequencing technology published M. makes possibility artifact less likely, our results from both PCR amplification followed by Sanger mapping based on whole-genome resequencing datasets suggested that containing possibly due to misassembly sequences. Our current highlights necessity validate authenticity identified previous searches assemblies.