Biosynthesis of glycosaminoglycans by cultured mastocytoma cells.

摘要: Biosynthesis of glycosaminoglycans by several lines cultured neoplastic mouse mast cells was studied incorporation [35S]sulphate (and in some cases [6-3H]glucosamine) into macromolecular materials found both the and their growth media. Such intracellular extracellular radioactively labelled (shown to be susceptibility digestion with heparinase) were further characterized ion-exchange chromatography testicular hyaluronidase chondroitinase. All but one cell line produced chondroitin sulphate as major sulphated glycosaminoglycan; remainder glycosaminoglycan heparin-like material. No [3H]hyaluronic acid synthesized. Cells a newly derived line, termed P815S, synthesized more than other lines. This glycosaminoglycan, medium, almost entirely 4-sulphate. 6-sulphate found. The 4-sulphate from shown gel filtration smaller media these cultures. discovery relatively high proportions mastocytoma-derived is noteworthy, since have generally been considered produce heparin glycosaminoglycan.