Peptide nanowires for coordination and signal transduction of peroxidase biosensors to carbon nanotube electrode arrays.

摘要: A strategy of metallizing peptides to serve as conduits electronic signals that bridge between a redox enzyme and carbon-nanotube electrode has been developed with enhanced results. In conjunction, protocol link the biological elements tips carbon nanotubes optimize contact geometry nanotube array. peptide nanowire 33 amino acids, comprised leucine zipper motif, was mutated bind divalent metals, conferring conductivity into peptide. Reaction thiolate sulfenic acid NADH peroxidase formed peptide-enzyme assembly are fully primed transduce electrons out active site an electrode. Scanning electron microscopy shows immobilization linking specifically electrodes, designed. Isothermal titration calorimetry mass spectrometry indicate binding stoichiometry at least three metals bound per strand. Overall, these results highlight gain can be achieved when signal tranducing units biosensor aligned through directed chemistry.