Leukocyte phagocytosis and lysozyme activity in Nile tilapia fed supplemented diet with natural extracts of propolis and Aloe barbadensis

作者: Geovana Dotta , Jaqueline Inês Alves de Andrade , Eduardo Luiz Tavares Gonçalves , Aline Brum , Jacó Joaquim Mattos

DOI: 10.1016/J.FSI.2014.05.020

关键词:

摘要: Although there is evidence on the benefits in use of immunostimulants aquaculture, are few commercial products being used. This study evaluated natural substances as potential sources for production immunostimulants. Propolis and Aloe barbadensis have been widely studied its extracts different chemical constituents responsible antimicrobial, anti-inflammatory immunostimulant. Tilapia juveniles were fed two weeks with diets supplemented mix propolis aloe (1:1) concentrations: 0.5, 1 e 2%. After experimental period, fish blood was collected hematoimmunological follows : hematocrit, total plasma protein, erythrocytes (RBC), leukocytes (WBC), differential leukocyte count, phagocytic activity, serum lysozyme antimicrobial activity (evaluated against Aeromonas hydrophila, Enterococcus durans Escherichia coli). Except higher number thrombocytes 1%-supplemented fish, rest did not show significant difference.

参考文章(42)
F. M. Lanças, J. F. T. Spencer, M. Maraschin, C. A. de Lacerda, A. L. Ragout de Spencer, M. Passos, J. D. Fontana, J. Adelmann, Propolis: chemical micro-heterogeneity and bioactivity. Environmental microbiology: methods and protocols. pp. 203- 218 ,(2004)
Paul B. Goldenfarb, Frank P. Bowyer, Elmer Hall, Effie Brosious, Reproducibility in the Hematology Laboratory: The Microhematocrit Determination American Journal of Clinical Pathology. ,vol. 56, pp. 35- 39 ,(1971) , 10.1093/AJCP/56.1.35
Daniela Avilez Duo, Rodrigo Yudi Fujimoto, Joaquim Goncalves Machado Neto, Claudinei da Cruz, Matheus Nicolino Peixoto Henares, Eficácia do paration metílico e do extrato aquoso de folhas secas de nim no controle de anacanthorus penilabiatus (monogenoidea) em pacu (piaractus mesopotamicus) Boletim Do Instituto De Pesca. ,vol. 34, pp. 61- 69 ,(2008)
Marcelo Maraschin, Amélia RS Zeggio, Ana Carolina V Souza, Antônio G Ferreira, Diego A da Silva, Flavia A Vieira, Ivanir Cella, Ivone Delgadillo, Maíra M Tomazzoli, Maria BR Veleirinho, Priscilla MM Lemos, Rúbia Mara Pinto, Shirley Kuhnen, Simone Kobe de Oliveira, Virgílio G Uarrota, None, Metabolomics and Chemometrics as Tools for Chemo(bio)diversity Analysis - Maize Landraces and Propolis InTech. ,(2012) , 10.5772/32584
Marjory B Brooks, Kendal E Harr, Davis M Seelig, K Jane Wardrop, Douglas J Weiss, None, Schalm's Veterinary Hematology ,(1986)
J.L.P. MOURIÑO, F. Do NASCIMENTO VIEIRA, A.B. JATOBÁ, B.C. Da SILVA, G.F.A. JESUS, W.Q. SEIFFERT, M.L. MARTINS, Effect of dietary supplementation of inulin and W. cibaria on haemato‐immunological parameters of hybrid surubim (Pseudoplatystoma sp) Aquaculture Nutrition. ,vol. 18, pp. 73- 80 ,(2012) , 10.1111/J.1365-2095.2011.00879.X
Pmm Lemos, DB Bach, PF Dias, LH Campestrini, M. Maraschin, S. Kuhnen, Cloning Protocol of Aloe vera as a Study-Case for “Tailor-Made” Biotechnology to Small Farmers Journal of Technology Management & Innovation. ,vol. 1, pp. 76- 79 ,(2006)
K Matsunaga, M Fujii, H Kobayashi, PSK as a chemopreventive agent. Cancer Epidemiology, Biomarkers & Prevention. ,vol. 2, pp. 271- 276 ,(1993)
Scott Peddie, Jun Zou, Christopher J Secombes, Immunostimulation in the rainbow trout (Oncorhynchus mykiss) following intraperitoneal administration of Ergosan Veterinary Immunology and Immunopathology. ,vol. 86, pp. 101- 113 ,(2002) , 10.1016/S0165-2427(02)00019-3
D. Christybapita, M. Divyagnaneswari, R. Dinakaran Michael, Oral administration of Eclipta alba leaf aqueous extract enhances the non-specific immune responses and disease resistance of Oreochromis mossambicus. Fish & Shellfish Immunology. ,vol. 23, pp. 840- 852 ,(2007) , 10.1016/J.FSI.2007.03.010