Innervation of the pigeon oviduct: correlation of NADPH diaphorase with acetylcholinesterase, tyrosine hydroxylase, and neuropeptides

摘要: Abstract The motility of the avian oviduct is controlled by hormones and neurons, but little microscopically known about a neural network in oviduct. present study was investigated to determine distribution nitric oxide-synthesizing neurons pigeon histochemistry for nicotinamide adenine dinucleotide phosphate-diaphorase (NADPH-d). NADPH-d reaction seen fibers. were mainly distributed around arterioles intermuscular tissue upper (infundibulum, magnum, isthmus); addition, also smooth muscle layers lamina propria lower (uterus vagina). found singly or small groups two–eight cell bodies. number smallest infundibulum, gradually increased toward vagina. shown be strongly positive many ganglia vaginal adventitia. Bundles fibers ran layer, surrounded blood vessels, connected with forming strands. Thin branched from these bundles constituted finer layer propria. Acetylcholinesterase staining showed similar pattern By double staining, 70∼77% colocalization acetylcholinesterase uterus Tyrosine hydroxylase immunoreactivity stained only nerve largely vessels Nerve immunoreactive calcitonin-gene related peptide, galanin, methionine-enkephalin, substance P, vasoactive intestinal peptide sparsely These results demonstrate that nitrergic make up large subpopulation intrinsic are closely associated cholinergic system oviduct, thus suggesting oxide acetylcholine could used modify relaxation