SILAC-MS Profiling of Reconstituted Human Chromatin Platforms for the Study of Transcription and RNA Regulation.

摘要: DNA packaged into chromatin is the core structure of human genome. Nearly all eukaryotic genome regulation must interface with this genomic structure, and modification can influence molecular mechanisms that regulate underlying DNA. Many processes are governed by regulated stepwise assembly build complex machinery on to license a specific activity such as transcription. Transcriptional activators drive initial steps gene expression, in part chromatin. Here we describe tools study protein complexes highly controlled manner using reconstituted platforms quantitative proteomic profiling. We profile early transcriptional activation highlight potential for understanding multiple ways regulation. also modifications approach long noncoding RNA act dynamic scaffold proteins be recruited This has provide more comprehensive important macromolecular occurs The nature substrate offers tunable system trapped at substeps understand how interfaces machinery.