AntisenseMBD2 gene therapy inhibits tumorigenesis
作者： Andrew Slack , Veronica Bovenzi , Pascal Bigey , M. A. Ivanov , Shyam Ramchandani
DOI: 10.1002/JGM.288
关键词:
摘要: Background Aberration in the pattern of DNA methylation is one hallmarks cancer. We present data suggesting that dysregulation MBD2, a recently characterized member novel family methylated binding proteins, involved tumorigenesis. Two functions were ascribed to demethylase activity and repression genes. Methods Multiple antisense expression delivery systems, transfection, electrotransfer adenoviral employed demonstrate MBD2 essential tumorigenesis, both ex vivo vivo. Results Inhibition by resulted inhibition anchorage-independent growth transfected cancer cells or infected with an vector expressing antisense. Xenograft tumors treated xenografts electrotransferred plasmids showed reduced growth. Conclusions These results support hypothesis described for are critical tumorigenesis potential anticancer target. Copyright © 2002 John Wiley & Sons, Ltd.
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