In Vivo Simultaneous Tracing and Ca2+ Imaging of Local Neuronal Circuits

摘要: A central question about the brain is how information processed by large populations of neurons embedded in intricate local networks. Answering this requires not only monitoring functional dynamics many simultaneously, but also interpreting such activity patterns context neuronal circuitry. Here, we introduce a versatile approach for loading Ca(2+) indicators vivo electroporation. With method, imaging can be performed both at neuron population level and with exquisite subcellular resolution down to dendritic spines axon boutons. This enabled mitral cell odor-evoked ensemble analyzed simultaneously revealing their specific connectivity different glomeruli. Colabeling Purkinje dendrites intersecting parallel fibers allowed presynaptic boutons postsynaptic dendrites. thus provides an unprecedented capability visualizing active ensembles tracing underlying circuits.