Genetic Manipulation of Plasmodium falciparum

摘要: This chapter reviews historical and technical issues, achievements to date, future possibilities with respect transformation of Plasmodium falciparum blood stages. Despite the relatively low efficiency P. transfection system, it was sufficient for both gene targeting transgene expression approaches be developed utilized analyze function in this organism. Given inability transfect linear DNA achieve fast integration into genome, a key success is somewhat mysterious ability transfected plasmids replicate episomally parasites. Transfection stages, using transient reporter genes chloramphenicol acetyl transferase luciferase, has been an important tool identification functional characterization number transcriptional control elements from Plasmid vectors designed express transgenes have used extensively falciparum, useful wide variety studies. A major advance understanding molecular dynamics cellular systems derived elegant studies which encoding green fluorescent protein (GFP) appended interest living cell. Since development berghei need inducible expression/conditional knockout system recognized as crucial next step allow analysis many stage genes.