Enhancement of the Aquaporin Adipose Gene Expression by a Peroxisome Proliferator-activated Receptor γ

摘要: The current study demonstrates that aquaporin adipose (AQPap), an adipose-specific glycerol channel (Kishida, K., Kuriyama, H., Funahashi, T., Shimomura, I., Kihara, S., Ouchi, N., Nishida, M., Nishizawa, Matsuda, Takahashi, Hotta, Nakamura, Yamashita, Tochino, Y., and Matsuzawa, Y. (2000) J. Biol. Chem. 275, 20896-20902), is a target gene of peroxisome proliferator-activated receptor (PPAR) gamma. AQPap mRNA amounts increased following the induction PPARgamma in differentiation 3T3-L1 adipocytes. tissue when mice were treated with pioglitazone (PGZ), synthetic ligand, decreased PPARgamma(+/-) heterozygous knockout mice. In adipocytes, PGZ augmented expression its promoter activity. Serial deletion revealed putative response element (PPRE) at -93/-77. preadipocytes, by transfection activated luciferase activity containing PPRE, whereas PPRE-deleted mutant was not affected. gel mobility shift assay showed direct binding PPARgamma-retinoid X alpha complex to PPRE. DeltaPPARgamma, which we generated as dominant negative lacking activation function-2 domain, suppressed cells, dose-dependently. We conclude novel through PPRE region promoter.