Poisoning by plant material: review of human cases and analytical determination of main toxins by high-performance liquid chromatography-(tandem) mass spectrometry.

摘要: The authors have reviewed the main toxic plants responsible for human deaths throughout world. Forty (genera or species) were listed in order to establish an inventory of active molecules that could be identified, already published analytical methods and reported fatal cases. In a second step, developed general method detection various toxins whole blood by high-performance liquid chromatography coupled mass spectrometry tandem spectrometry. Sample preparation was realized liquid-liquid extraction at pH 9.5 oleandrine, taxol alkaloids. These latter compounds divided into two groups following their chemical properties subsequently purified acid/base clean up. Cyanogenic atractyloside isolated precipitation protein content with acetone washing chloroform. Separation drugs occurred under reversed-phase conditions on C18 column 150x2 mm I.D. (5 microm particle size) using different mobile phases. first one, formiate buffer 2 mM acidified 3.0, used separation atractyloside, taxol, cyanogenic some phase, 10 made basic 8.2 majority other A gradient elution mode chosen acetonitrile acetonitrile-methanol (50:50, v/v) as eluting solvent. Detection positive ionization choice all except (negative ions) (mixed available). Application real forensic cases has been demonstrated.