Neutral Red Uptake and Clonogenic Survival Assays of the Hyperthermic Sensitization of Tumor Cells to Tumor Necrosis Factor

摘要: Vital dye uptake and postfixation assays have recently been used to examine the interaction between short-term (24-48 h) exposures monokine, tumor necrosis factor (TNF), hyperthermic treatments with finding that synergistic increases in cytotoxicity occurred. However, survival measured by these is not necessarily closely related eventual loss of clonogenic capacity. Treatment-induced growth delays, delayed cytotoxic effects, or perturbations vital sequestration mechanisms could result a different measurement surviving fraction than given assay. In this study we directly compared neutral red confirmed both TNF-sensitive (L-929) TNF-resistant (EMT-6) phenotypes show greatly reduced when treated combined recombinant human TNF (1.0-0.0005 μg/ml) hyperthermia (1-2 h at 43°C). Moreover, sensitization EMT-6 cel...