Controlled Release of Thymosin Beta 4 Using a Collagen–Chitosan Sponge Scaffold Augments Cutaneous Wound Healing and Increases Angiogenesis in Diabetic Rats with Hindlimb Ischemia

摘要: It is important to establish an efficient vascularization for the long-term acceptance of bioengineered skin equivalents treating cutaneous wounds diabetic rats with hindlimb ischemia. This study investigates possible use a collagen–chitosan sponge scaffold encapsulated thymosin beta 4 (CCSS-eTβ4), angiogenic factor, accelerate wound healing in streptozotocin (STZ)-induced CCSSs-eTβ4 was fabricated using freeze-drying method. The scaffolds were analyzed by scanning electron microscopy, swelling and degradation assays, mechanical properties, 50:50 selected applied. controlled release Tβ4 from elicited localized prolonged effects over 12 days, as shown enzyme-linked immunosorbent assay (ELISA). In vivo, improved healing, faster reepithelialization, better dermal reorganization, higher vascularization. Fu...