Intracellular antimicrobial photodynamic therapy: a novel technique for efficient eradication of pathogenic bacteria.

摘要: The increasing resistance of bacteria to antibiotics is a serious problem, caused in part by excessive and improper use these drugs. One alternative traditional antibiotic therapy photodynamic antimicrobial chemotherapy (PACT) which based on the photosensitizer (PS), activated illumination with visible light. poor penetration light through skin limits application PACT treatment infections or invasive procedures. To overcome this problem we report exploitation emitted as result chemiluminescent reaction luminol excite PS call process (CPAT). We studied effect free liposome-encapsulated (methylene blue toluidine blue) under excitation either white external chemiluminescence liposome-enclosed luminol. showed slightly better performance that CPAT for encapsulated both types bacteria. resulted three log suppression Staphylococcus aureus two Escherichia coli growth. may prove be novel more effective form therapy, particularly internal not easily accessible PACT.