A high-throughput screening procedure for enhancing α-ketoglutaric acid production in Yarrowia lipolytica by random mutagenesis

摘要: Abstract Yarrowia lipolytica is one of the most intensively investigated α-ketoglutaric acid (α-KG) producers. A series studies have been carried out, but enhancing production via application metabolic engineering and genetic associated with potential safety concerns in some countries regions. In this study, a high-throughput screening procedure was established to solve problem by an atmospheric room temperature plasma based random mutagenesis process. This method implemented on use pH indicators because changes during fermentation For pre-screening procedure, indicator bromocresol green, which sensitive cannot alter growth microorganisms culture plate, applied exclude negative mutants. multi-cell-plate process, six different were tested. Among them, quinaldine red performed best. Based mutant (1-C6) successfully obtained from 2880 The titers α-KG produced 1-C6 enhanced 51.8% 45.4% compared those wild type 500-mL shake flasks 3-L fermenter, respectively. process here could well facilitate other similar organic acids or evolutionary processes.