Spatial organization and isotubulin composition of microtubules in epidermal tendon cells of Artemia franciscana.

摘要: Epidermally derived tendon cells attach the exoskeleton (cuticle) of Branchiopod crustacean, Artemia franciscana, to underlying muscle in hindgut, while structurally similar transalar (epithelial) cells, which also arise from epidermis and are polarized, connect dorsal ventral exopodite surfaces. To establish these latter attachments interact with cuticles on opposite sides by way their apical surfaces one another via basal regions, or cuticle may be mediated through linkages phagocytic storage found hemolymph. In some cases, phyllopod directly cells. Tendon hindgut possess microtubule bundles, as do they extend myotendinal junction domain located near cuticle. The bundled microtubules intermingle thin filaments reminiscent microfilaments, but intermediate filament-like structures absent. Microtubule bundles converging at cell contact termed invaginations, composed cytoplasmic membrane infoldings associated electron-dense material. Intracuticular rods protrude either into during intermolt molting fluid premolt. Confocal microscopy immunofluorescently stained samples revealed tyrosinated, detyrosinated, acetylated tubulins, first time posttranslationally modified isoforms this protein have been demonstrated crustacean Microfilaments, shown staining phalloidin, coincided spatially bundles. clearly represent an interesting system for study cytoskeleton organization within context polarity results article indicate functional cooperation microfilaments. These cytoskeletal elements, acting independently concert, transmit tension resist compression when connecting cuticular © 2004 Wiley-Liss, Inc.