Detection of Yersinia pestis DNA in prairie dog-associated fleas by polymerase chain reaction assay of purified DNA.
作者： Karen A. Griffin , Daniel J. Martin , Laura E. Rosen , Michael A. Sirochman , Daniel P. Walsh
DOI: 10.7589/0090-3558-46.2.636
关键词:
摘要: We evaluated, refined, and applied well-established polymerase chain reaction (PCR) techniques for detecting Yersinia pestis DNA in fleas (mainly Oropsylla spp.) collected from prairie dog (Cynomys burrows. Based on results PCR of avirulent Y. strain A1122 DNA, we used purification primers the plasminogen activator gene to screen field-collected fleas. detected flea pools two black-tailed ludovicianus) colonies with evidence recent plague epizootics, one four white-tailed leucurus) colony complexes (Wolf Creek) where epizootic was lacking. Relative abundance occurrence among appeared vary over time at Wolf Creek. Both primer sequences influence likelihood by burrows absence observed plague. Presence pla...
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