Isotope labeling in insect cells.
作者： Krishna Saxena , Arpana Dutta , Judith Klein-Seetharaman , Harald Schwalbe
DOI: 10.1007/978-1-61779-480-3_3
关键词:
摘要: Recent years have seen remarkable progress in applying nuclear magnetic resonance (NMR) spectroscopy to proteins that traditionally been difficult study due issues with folding, posttranslational modification, and expression levels or combinations thereof. In particular, insect cells proved useful allowing large quantities of isotope-labeled, functional be obtained purified homogeneity, their structures dynamics by using NMR. Here, we provide protocols proven successful such endeavors.
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