Investigation of the Primary Photochemistry of Bacteriorhodopsin by Low‐Temperature Fourier‐Transform Infrared Spectroscopy
作者： Friedrich SIEBERT , Werner MANTELE
DOI: 10.1111/J.1432-1033.1983.TB07187.X
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摘要: The method of Fourrier-transform infrared difference spectroscopy was applied to investigate the transition at 77K bacteriorhodopsin in its light-adapted form K610, first intermediate which is stable low temperature. In addition unmodified bacteriorhodopsin, 2H2O and containing [15-2H]retinal used. results show that major rearrangements occur Schiff base this transition. It not possible identify a C=N stretching vibration K610. identification an N-H bending K610 shows nitrogen previous still has proton attached. fingerprint region exhibits very unusual features for bears no similarity protonated retinylidene model compounds any isomeric composition. Therefore, conclusions on state retinal can be drawn. spectra terminal part predominantly reflected spectra. This indicates most polar located near base. We have evidence protein molecular changes occurring 77K.
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