Differential DNA methylation and transcription profiles in date palm roots exposed to salinity.

摘要: As a salt-adaptive plant, the date palm (Phoenix dactylifera L.) requires suitable mechanism to adapt stress of saline soils. There is growing evidence that DNA methylation plays an important role in regulating gene expression response abiotic stresses, including salinity. Thus, present study sought examine differential status occurs genome when plants are exposed salinity, and identify salinity responsive genes regulated by methylation. To achieve these, whole-genome bisulfite sequencing (WGBS) was employed mRNA sequenced from salinity-treated untreated roots. The WGBS analysis included 324,987,795 317,056,091 total reads control samples, respectively. covered about 81% genomic with 40% mapping efficiency average read depth 17-fold coverage per strand, conversion rate around 99%. level within differentially methylated regions (DMRs) significantly (p < 0.05, FDR ≤ 0.05) increased specifically at mCHG mCHH sequence contexts. Consistently, mass spectrometry enzyme-linked immunosorbent assay (ELISA) showed there significant increase global revealed presence 6,405 value 0.001, Integration high-resolution methylome transcriptome analyses negative correlation between mCG located promoters expression, while positive noticed mCHG/mCHH rations grew under conditions. Therefore, relationships vary based on context, region gene, protein-coding ability treatment. These results provide insights into interplay among highlight effect nature this relationship, which may involve other genetic epigenetic players salt obtained project first draft map stress.