Milliscale Substrate Curvature Promotes Myoblast Self-Organization and Differentiation.

摘要: Biological tissues comprise complex structural environments known to influence cell behavior via multiple interdependent sensing and transduction mechanisms. Yet, despite the predominantly nonplanar geometry of these environments, impact tissue-size (milliscale) curvature on is largely overlooked or underestimated. This study explores how concave, hemicylinder-shaped surfaces 3-50 mm in diameter affect migration, proliferation, orientation, differentiation C2C12 myoblasts. Notably, milliscale cues significantly responses compared with planar substrates, myoblasts grown 7.5-15 mm showing prevalent migration alignment parallel axis. Moreover, within this range promote myoblast formation denser, more compact comprising highly oriented multinucleated myotubes. Based similarity effects, it further proposed that susceptibility substrate depends mechanotransduction signaling. model thus supports notion cellular compliance share same molecular pathways control can be achieved modulation either individual parameter combination. correlation relevant for elucidating muscle tissue forms heals, as well designing better biomaterials appropriate cell-surface interfaces.