A biochemical and kinetic analysis of the interleukin-1 receptor. Evidence for differences in molecular properties of IL-1 receptors.
作者： R Horuk , JJ Huang , M Covington , RC Newton
DOI: 10.1016/S0021-9258(18)49250-2
关键词:
摘要: This study describes the biochemical characterization and kinetic analysis of interleukin-1 (IL-1) receptor in Raji human B-lymphoma EL4 murine T-lymphoma cells. The internalization 125I-IL-1 was studied both cell types by an acid extraction technique which removes surface bound ligand. At 37 degrees C, binding to IL-1 receptors almost entirely (91%). cells, contrast, internalized 59% ligand at this temperature totally inhibited sodium azide. Receptor studies showed that B-cells had a lower affinity but much higher density per (KD = 2.1 nM, Ro 7709) than T-cells 0.4 241). two analogs, Glu-4 clone 18, determined competitive studies. In analogs affinities 25 90%, respectively, whereas were 0.2 200%, respectively. Chemical cross-linking molecular weight (68 kDa compared 80 kDa). summary these demonstrate structural differences exist between
sci-hub.st 参考文章(23)
Steven K. Dower, Shirley R. Kronheim, Thomas P. Hopp, Michael Cantrell, Michael Deeley, Steven Gillis, Christopher S. Henney, David L. Urdal, The cell surface receptors for interleukin-1α and interleukin-1β are identical Nature. ,vol. 324, pp. 266- 268 ,(1986) , 10.1038/324266A0
H.T. Haigler, F.R. Maxfield, M.C. Willingham, I. Pastan, Dansylcadaverine inhibits internalization of 125I-epidermal growth factor in BALB 3T3 cells. Journal of Biological Chemistry. ,vol. 255, pp. 1239- 1241 ,(1980) , 10.1016/S0021-9258(19)86019-2
U Gubler, A S Stern, J J Farrar, P L Kilian, D Woehle, P T Lomedico, K L Kaffka, T M Dechiara, W R Benjamin, S B Mizel, Interleukin 1 alpha and interleukin 1 beta bind to the same receptor on T cells. Journal of Immunology. ,vol. 136, pp. 4509- 4514 ,(1986)
G.A McPherson, A practical computer-based approach to the analysis of radioligand binding experiments Computer Programs in Biomedicine. ,vol. 17, pp. 107- 113 ,(1983) , 10.1016/0010-468X(83)90031-4
Philip L. Simon, Jeffrey T. Laydon, John C. Lee, A modified assay for interleukin-1 (IL-1) Journal of Immunological Methods. ,vol. 84, pp. 85- 94 ,(1985) , 10.1016/0022-1759(85)90417-X
H. Bourne, P Coffino, G. Tomkins, Selection of a variant lymphoma cell deficient in adenylate cyclase Science. ,vol. 187, pp. 750- 752 ,(1975) , 10.1126/SCIENCE.163487
George Scatchard, The Attractions of Proteins for Small Molecules and Ions Annals of the New York Academy of Sciences. ,vol. 51, pp. 660- 672 ,(1949) , 10.1111/J.1749-6632.1949.TB27297.X
S. K. Dower, S. M. Call, S. Gillis, D. L. Urdal, Similarity between the interleukin 1 receptors on a murine T-lymphoma cell line and on a murine fibroblast cell line Proceedings of the National Academy of Sciences of the United States of America. ,vol. 83, pp. 1060- 1064 ,(1986) , 10.1073/PNAS.83.4.1060
T. A. Bird, J. Saklatvala, Identification of a common class of high affinity receptors for both types of porcine interleukin-1 on connective tissue cells Nature. ,vol. 324, pp. 263- 266 ,(1986) , 10.1038/324263A0
S K Durum, J A Schmidt, J J Oppenheim, Interleukin 1: An Immunological Perspective Annual Review of Immunology. ,vol. 3, pp. 263- 287 ,(1985) , 10.1146/ANNUREV.IY.03.040185.001403