Effects of substrate and ectomycorrhizal inoculation on the development of two-years-old container-grown Norway spruce (Picea abies Karst.) seedlings

摘要: Abstract: The objective of this study was to test the effects selected peat growth substrates (Agro CS, Gramoflor and Durpeta) inoculation with commercial ectomycorrhizal inocula (Ectovit Mycorrhizaroots) on growth, ectomycorrhiza formation, needle nutrients concentration several physiological parameters two-years-old containerized Norway spruce seedlings cultivated under standard nursery conditions. differed in origin, composition nutrient content: Agro CS were mixtures various types components added nutrients, while Durpeta non-enriched pure peat. Growth enriched significantly higher than those grown substrate. Significant interactions found between substrate treatments. Inoculation Ectovit stimulated seedling but had no effect substrates, a negative aboveground biomass Gramoflor. Mycorrhizaroots inoculum decreased shoot root dry weight ratio, other development. ECM colonization rate ranged from 73 80%, significant or DNA analysis revealed low species diversity fungi roots, pronounced dominance soil-borne Thelephora terrestris Fr. Chemical needles measurement chlorophyll fluorescence showed similar trends as growth. Values N, P, K, Ca Mg concentrations both substrates. increased (though not significantly) compared Mycorrhizaroots- non-inoculated seedlings, well nutrient-uptake (mainly K) Our results suggest importance origin peat-based development container-grown observed positive more probably caused by its physical chemical properties rather efficiency promoting symbiosis. tested appear be suitable for production acceptable size outplanting within two growing seasons.