Association and Folding of Small Oligomeric Proteins

摘要: Originally published in: Protein Folding Handbook. Part I. Edited by Johannes Buchner and Thomas Kiefhaber. Copyright © 2005 Wiley-VCH Verlag GmbH & Co. KGaA Weinheim. Print ISBN: 3-527-30784-2 The sections in this article are Introduction Experimental Methods Used to Follow the of Oligomeric Proteins Equilibrium Methods Kinetic Methods Dimeric Proteins Two-state Dimeric Proteins Examples Proteins Obeying Two-state Folding Folding through Intermediate States Trimeric Tetrameric Proteins Conclusions Appendix–Concurrent Association Small Constants for Folding Homooligomeric Protein Heterooligomeric Protein Calculation Thermodynamic Parameters from Equilibrium Constants Basic Relationships Linear Extrapolation Denaturant Unfolding Curves Reaction Calculation van't Hoff Enthalpy Change Thermal Data Calculation Concentration-dependence Tm Extrapolation Different Temperatures: Gibbs-Helmholtz Equation Kinetics Reversible Unfolding: Integrated Rate Equations Two-state Protein Two-state Protein Reversible Unfolding Homodimeric protein Heterodimeric protein Kinetics Folding: Relaxation after Disturbance a Preexisting (Method Bernasconi) Acknowledgments Keywords: protein folding; dimeric proteins; oligomeric proteins; two-state folding; leucine zipper; folding intermediates