Specific knockdown of the D2 Long dopamine receptor variant

摘要: Dopamine signaling in the nucleus accumbens is critical mediating effects of cocaine. There are two splice variants dopamine D2 receptors, D2L and D2S, which believed to have different functional roles. Here we show that knocking down selectively using viral mediated shRNA led a slight but significant decrease basal locomotor activity with no change cocaine induced stimulation locomotion. The knockdown appears produce trend reduced conditioned place preference difference was not statistically significant. Our results demonstrated receptors can be manipulated vivo specific brain regions allowing more studies each receptor isoform.