MTA2 enhances colony formation and tumor growth of gastric cancer cells through IL-11

摘要: We have preliminarily reported MTA2 expression in gastric cancer and its biological functions by using knockdown cell models, while the molecular mechanisms of regulating malignant behaviors are still unclear. overexpression models were established transfection assay cells BGC-823 MKN28. Cell proliferation assay, colony formation soft agar, wound-healing transwell migration performed with negative control (NC) cells. Subcutaneous xenografts pulmonary metastasis BGC-823/MTA2 BGC-823/NC used to observe capacity growth vivo. Differential gene was analyzed microarrays. IL-11, which demonstrated as differential microarray, detected real-time PCR, western blot, ELISA immunohistochemistry staining. Recombinant human IL-11 (rhIL-11) administrated rescue assay. The numbers colonies agar significantly more MKN28/MTA2 cells, comparing those their NC Capabilities proliferation, not changed sizes subcutaneous metastases BGC-832/MTA2 larger than group. identified genome microarray both elevated whereas reduced SGC-7901/shMTA2 Administration rhIL-11 recovered enhances tumor but plays important role metastasis. is one downstream effectors growth.