Isozymes of S-adenosylmethionine synthetase from rat liver: isolation and characterization.

摘要: S-Adenosylmethionine synthetase exists in at least two distinct forms, alpha- and beta-forms, adult liver. The beta-form was purified to homogeneity from the soluble fraction of rat liver with a yield about 10%. An antiserum directed against prepared by injecting enzyme into rabbit. Ouchterlony double diffusion analysis immunochemical titrations revealed that isozymes, are identical. Thus, alpha-form isolated as single protein using immunoaffinity chromatography beta-form. molecular weights beta- alpha-forms were determined be 48,000 each sodium dodecyl sulfate disc gel electrophoresis, 100,000, 200,000, respectively, Sephacryl S-200 filtration. These results indicate consisted subunits daltons four daltons. sedimentation coefficient calculated 5.5S for 8.0S alpha-form.