Serodiagnosis of Louse-Borne relapsing fever with glycerophosphodiester phosphodiesterase (GlpQ) from Borrelia recurrentis.

摘要: Human louse-borne relapsing fever occurs in sporadic outbreaks central and eastern Africa that are characterized by significant morbidity mortality. Isolates of the causative agent, Borrelia recurrentis, were obtained from blood four patients during a recent epidemic disease southern Sudan. The glpQ gene, encoding glycerophosphodiester phosphodiesterase, these isolates was sequenced compared with sequences other relapsing-fever spirochetes. Previously we showed GlpQ hermsii is an immunogenic protein utility as serological test antigen for discriminating tick-borne Lyme disease. In present work, cloned expressed gene B. recurrentis used recombinant tests. Acute- convalescent-phase serum samples 42 tested indirect immunofluorescence assay (IFA) enzyme-linked immunosorbent (ELISA) whole cells immunoblotting to whole-cell lysates spirochete Escherichia coli producing GlpQ. geometric mean titers acute- measured IFA 1:83 1:575, respectively. immunoblot analysis identified high level reactivity seroconversion GlpQ, more sensitive than ELISA using purified, histidine-tagged Serum antibodies antigens persisted 27 years one patient. We conclude assessment anti-GlpQ will allow confirmation determination prevalence.