A deep profiler's guide to cytometry

摘要: In recent years, advances in technology have provided us with tools to quantify the expression of multiple genes individual cells. The ability measure simultaneously same cell is necessary resolve great diversity subsets, as well define their function host. Fluorescence-based flow cytometry benchmark for this; it, we can 18 proteins per cell, at >10 000 cells/s. Mass a new that promises extend these capabilities significantly. Immunophenotyping by mass spectrometry provides >36 rate 1000 We review cytometric technologies, capable high-content, high-throughput single-cell assays.