Escin activates AKT-Nrf2 signaling to protect retinal pigment epithelium cells from oxidative stress.
作者： Kaijun Wang , Yiqian Jiang , Wei Wang , Jian Ma , Min Chen
DOI: 10.1016/J.BBRC.2015.10.117
关键词:
摘要: Here we explored the anti-oxidative and cytoprotective potentials of escin, a natural triterpene-saponin, against hydrogen peroxide (H2O2) in retinal pigment epithelium (RPE) cells. We showed that escin remarkably attenuated H2O2-induced death apoptosis established (ARPE-19) primary murine RPE Meanwhile, ROS production lipid peroxidation by H2O2 were inhibited escin. Escin treatment cells resulted NF-E2-related factor 2 (Nrf2) signaling activation, evidenced transcription anti-oxidant-responsive element (ARE)-regulated genes, including HO-1, NQO-1 SRXN-1. Knockdown Nrf2 through targeted shRNAs/siRNAs alleviated escin-mediated ARE gene transcription, almost abolished anti-oxidant activity cytoprotection H2O2. Reversely, was more potent damages Nrf2-over-expressed ARPE-19 Further studies escin-induced activation required AKT signaling. inhibitors (LY294002 perifosine) blocked dramatically phosphorylation, its cytosol accumulation nuclear translocation Escin-induced also inhibitors. Together, these results demonstrate protects from oxidative stress possibly activating AKT-Nrf2
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