Towards deconvoluting the interaction of the Bacillus subtilis sporulation proteins SinR and SinI using tryptophan analogue incorporated proteins
作者： D. J. Scott , S. Leejeerajumnean , J. A. Brannigan , R. J. Lewis , A. J. Wilkinson
关键词:
摘要: Sporulation in Bacillus subtilis is used as the strategy of last resort for survival organism and it a very tightly controlled developmental process. One control checkpoints that must be overcome sporulation to occur repression genes by protein SinR (13.5 kDa). This done binding anti-repressor SinI (6.5 kDa) form bound heterodimer. To investigate interaction with solution, an analytical ultracentrifuge study was undertaken. found tetramer, whereas monomer/dimer equilibrium. Derivatives both SinR, where native tryptophan replaced analogue 7-aza-tryptophan (7AW), were expressed active wild-type proteins. The 7AW proteins have property having significant absorbance at 315 nm, thus allowing them monitored even presence containing proteins, making ideal studying protein/protein interactions.
springer.com
sci-hub.st 参考文章(16)
D. J. Scott, A. L. Ferguson, M. Buck, M. -T. Gallegos, M. Pitt, J. G. Hoggett, Use of the tryptophan analogue 7-azatryptophan to study the interaction of σN with Escherichia coli RNA polymerase core enzyme Progress in colloid and polymer science. pp. 212- 215 ,(1999) , 10.1007/3-540-48703-4_32
J.B. Alexander Ross, Arthur G. Szabo, Christopher W.V. Hogue, Enhancement of protein spectra with tryptophan analogs: fluorescence spectroscopy of protein-protein and protein-nucleic acid interactions. Methods in Enzymology. ,vol. 278, pp. 151- 190 ,(1997) , 10.1016/S0076-6879(97)78010-8
Arthur Jennison Rowe, S. E. Harding, J. C. Horton, Analytical ultracentrifugation in biochemistry and polymer science Royal Society of Chemistry. ,(1992)
Harry Svensson, The Second-Order Aberrations in the Interferometric Measurement of Concentration Gradients Journal of Modern Optics. ,vol. 1, pp. 164- 183 ,(1954) , 10.1080/713823673
Sandhya Callaci, Tomasz Heyduk, Conformation and DNA binding properties of a single-stranded DNA binding region of sigma 70 subunit from Escherichia coli RNA polymerase are modulated by an interaction with the core enzyme. Biochemistry. ,vol. 37, pp. 3312- 3320 ,(1998) , 10.1021/BI972041M
Cing-Yuen Wong, Maurice R. Eftink, Incorporation of Tryptophan Analogues into Staphylococcal Nuclease: Stability toward Thermal and Guanidine-HCl Induced Unfolding† Biochemistry. ,vol. 37, pp. 8947- 8953 ,(1998) , 10.1021/BI971863G
Donald J. Winzor, Michael P. Jacobsen, Peter R. Wills, Direct analysis of sedimentation equilibrium distributions reflecting complex formation between dissimilar reactants. Biochemistry. ,vol. 37, pp. 2226- 2233 ,(1998) , 10.1021/BI972211V
Christopher W.V. Hogue, Arthur G. Szabo, Characterization of aminoacyl-adenylates in B. tsubtilis tryptophanyl-tRNA synthetase, by the fluorescence of tryptophan analogs 5-hydroxytryptophan and 7-azatryptophan Biophysical Chemistry. ,vol. 48, pp. 159- 169 ,(1993) , 10.1016/0301-4622(93)85007-5
Richard Losick, Patrick Stragier, Crisscross regulation of cell-type-specific gene expression during development in B. subtilis. Nature. ,vol. 355, pp. 601- 604 ,(1992) , 10.1038/355601A0
Elena Rusinova, J. B. Alexander Ross, Thomas M. Laue, Lawrence C. Sowers, Donald F. Senear, Linkage between operator binding and dimer to octamer self-assembly of bacteriophage lambda cI repressor. Biochemistry. ,vol. 36, pp. 12994- 13003 ,(1997) , 10.1021/BI971368K