Comprehensive characterization and simultaneous analysis of overall lipids in reconstructed human epidermis using NPLC/HR-MSn: 1-O-E (EO) Cer, a new ceramide subclass.

摘要: Stratum corneum lipids are responsible for the skin's barrier function. They final product of epidermis lipid biosynthesis. During this process, evolve from simple to complex structures in three main levels respectively (stratum basal level, stratum granulosum and level). Our aim was simultaneously analyze characterize structure total lipids. A powerful analytical method (normal-phase liquid chromatography coupled with high-resolution mass spectrometry (NPLC/HR-MSn)) developed order separate, a single run, classes wide polarity range. Chromatographic conditions were particularly designed intermediate such as ceramides. Rich information obtained about molecular keratinocyte differentiation biomarkers ceramides, glucosylceramides, sphingomyelins microstructures reconstructed human using HR-MSn. new subclass 1-O-Acyl Omega-linoleoyloxy ceramides [1-O-E (EO) Cer] has been highlighted. This class is double esterified on 1-O-position sphingoid base long very chain acyl residues (1-O-E) position ω-hydroxyl group fatty acid linolenic (EO). Considering its chemical hydrophobicity, can contribute skin barrier. In addition, we detected sphingomyelins. lipidomic approach offers direct access biomarkers.