Differential expression of profibrotic and growth factors in chronic allograft nephropathy.

摘要: BACKGROUND Chronic allograft nephropathy (CAN) is a multifactorial process, where both immunological and nonimmunological factors play roles. Microarrays detect thousands of genes simultaneously. METHODS We have analyzed gene expression profiles 16 kidney transplant biopsy samples with CAN by high-density oligonucleotide microarrays, comparing to six normal biopsies. Eight biopsies showed nodular arteriolar hyalinization one was positive for C4d staining. RESULTS Hierarchical clustering analysis the 22 revealed differential patterns in versus control However, microarray did not reveal patients or without hyalinization. Fifty percent 100 highest hybridization intensities sample were related cellular humoral immune response. Although 212 upregulated minimum 1.5-fold, 112 downregulated samples. There profibrotic growth that while transforming factor-beta induced factor, thrombospondin 1, platelet derived factor-C up-regulated, vascular endothelial epidermal fibroblast 1 9 downregulated. Selected differentially expressed confirmed microdissected real-time quantitative PCR. Immunopathologic examination strong TGF-beta but decreased glomerular VEGF CAN. CONCLUSION might be an important tool uncover mechanisms diseases, such as