Lipidomic metabolism analysis of the endogenous cannabinoid anandamide (N-arachidonylethanolamide)
作者： Ekaterina A. Placzek , Bruce R. Cooper , Andrew T. Placzek , Julia A. Chester , V. Jo Davisson
DOI: 10.1016/J.JPBA.2010.03.035
关键词:
摘要: Elucidation of pathways involved with lipid metabolism has been limited by analytical challenges associated detection and structure identification. A discovery-based mass spectrometry lipidomic approach applied to identify metabolites the endogenous cannabinoid anandamide (N-arachidonylethanolamide). Previously, a model system was established show that can be recycled cells form new endocannabinoids suggesting recycling arachidonate carbon chain. We hypothesized distinct cellular exist direct anandamide-derived chain into specific set metabolites, different from metabolite pool is comprised non-anandamide-derived arachidonic acid. Using stable isotope encoding liquid chromatography-mass spectrometry, we identified derived exogenous or acid in RBL-2H3 cells. discovered acid-derived were primarily eicosanoid class, whereas acid, addition eicosanoids, metabolized diradylglycerols, fatty amides, sterols, glycerophospholipids. From list particular interest 1-O-arachidonyl-sn-glycero-3-phosphocholine. Furthermore, determined while 1-O-arachidonyl-sn-glycero-3-phosphocholine may anandamide, sn-2 compound more abundant mouse brain tissue. Overall, our results provide novel study metabolic fate signaling molecules.
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