Chromatographic characterisation of 11 phytocannabinoids: Quantitative and fit-to-purpose performance as a function of extra-column variance

摘要: Introduction Cannabis sativa L. (cannabis) is utilised as a therapeutic and recreational drug. With the legalisation of cannabis in many countries anticipated regulation potency that will accompany legalisation, analytical testing facilities require broadly applicable, quantitative, high throughput method to meet increased demand. Current methods for biologically active components (phytocannabinoids) suffer from low and/or an incomplete complement relevant phytocannabinoids. Objective To develop rapid, quantitative applicable liquid chromatography-tandem mass spectrometry 11 phytocannabinoids with acidic neutral character. Methodology Bulk diffusion coefficients were calculated using Taylor-Aris open tubular method, four reference compounds used validate experimental set-up. Three columns quantitatively evaluated van Deemter plots fit-to-purpose performance metrics. Low (1.2 μL2 ) standard (3.6 extra-column variance ultra-high pressure chromatography (UPLC) configurations contrasted. Method was demonstrated methanolic flower extracts. Results are reported. The developed chromatographic includes challenging Δ8 /Δ9 -tetrahydrocannabinol isobars and, at 6.5 min, faster than existing targeting similar panels Conclusions bulk curves informed development rapid facilitate potential expansion include additional compounds, including synthetic cannabinoids. can be implemented or UPLC configurations.