CHARACTERIZATION OF THE BINDING SITE FOR CYCLOTHIALIDINE ON THE B SUBUNIT OF DNA GYRASE
作者： Naoki Nakada , Hans Gmünder , Takahiro Hirata , Mikio Arisawa
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摘要: The mechanism of inhibition DNA gyrase by cyclothialidine, a novel inhibitor isolated from Streptomyces filipinensis NR0484, has been studied further using [14C]benzoylcyclothialidine and reconstituted Escherichia coli system consisting the A subunit, B subunit relaxed ColE1 DNA. was also with 43-kDa N-terminal fragment subunit. could bind to alone but not nor plasmid alone. Furthermore, compound bound Scatchard analysis binding showed that affinity increased, depending on assembly (A2B2)·DNA complex. This suggests site cyclothialidine or its vicinity causes conformational change during gyrase·DNA complex (increase in affinity: → A2B2 A2B2·DNA). displacement curves nonlabeled ATP analogues, coumarin antibiotics indicated coumarins, share common (or overlapping) action gyrase; however, microenvironment sites may differ.
sci-hub.se 参考文章(31)
Anthony Maxwell, Martin Gellert, Mechanistic Aspects of DNA Topoisomerases Advances in Protein Chemistry. ,vol. 38, pp. 69- 107 ,(1986) , 10.1016/S0065-3233(08)60526-4
Richard Otter, Nicholas R. Cozzarelli, Escherichia coli DNA gyrase. Methods in Enzymology. ,vol. 100, pp. 171- 180 ,(1983) , 10.1016/0076-6879(83)00053-1
L L Shen, W E Kohlbrenner, D Weigl, J Baranowski, Mechanism of quinolone inhibition of DNA gyrase. Appearance of unique norfloxacin binding sites in enzyme-DNA complexes. Journal of Biological Chemistry. ,vol. 264, pp. 2973- 2978 ,(1989) , 10.1016/S0021-9258(19)81708-8
R J Reece, A Maxwell, Tryptic fragments of the Escherichia coli DNA gyrase A protein. Journal of Biological Chemistry. ,vol. 264, pp. 19648- 19653 ,(1989) , 10.1016/S0021-9258(19)47162-7
K Mizuuchi, M Mizuuchi, M H O'Dea, M Gellert, Cloning and simplified purification of Escherichia coli DNA gyrase A and B proteins. Journal of Biological Chemistry. ,vol. 259, pp. 9199- 9201 ,(1984) , 10.1016/S0021-9258(17)47284-X
A Maxwell, M Gellert, The DNA dependence of the ATPase activity of DNA gyrase. Journal of Biological Chemistry. ,vol. 259, pp. 14472- 14480 ,(1984) , 10.1016/S0021-9258(17)42623-8
N Nakada, H Gmunder, T Hirata, M Arisawa, Mechanism of inhibition of DNA gyrase by cyclothialidine, a novel DNA gyrase inhibitor. Antimicrobial Agents and Chemotherapy. ,vol. 38, pp. 1966- 1973 ,(1994) , 10.1128/AAC.38.9.1966
Richard J. Lewis, Onkar M.P. Singh, Clare V. Smith, Anthony Maxwell, Tadeusz Skarzynski, Alan J. Wonacott, Dale B. Wigley, Crystallization of inhibitor complexes of an N-terminal 24 kDa fragment of the DNA gyrase B protein. Journal of Molecular Biology. ,vol. 241, pp. 128- 130 ,(1994) , 10.1006/JMBI.1994.1480
Richard J. Reece, Anthony Maxwell, The C-terminal domain of the Escherichia coli DNA gyrase A subunit is a DNA-binding protein Nucleic Acids Research. ,vol. 19, pp. 1399- 1405 ,(1991) , 10.1093/NAR/19.7.1399
Andrew P. Jackson, Anthony Maxwell, Dale B. Wigley, Preliminary crystallographic analysis of the ATP-hydrolysing domain of the Escherichia coli DNA gyrase B protein. Journal of Molecular Biology. ,vol. 217, pp. 15- 17 ,(1991) , 10.1016/0022-2836(91)90606-7