Degradation of 2,6-di-tert-butylphenol by an isolated high-efficiency bacterium strain

摘要: An aerobic bacterium strain, F-3-4, capable of effectively degrading 2, 6-ditert-butylphenol (2, 6-DTBP), was isolated and screened out from an acrylic fiber wastewater the biofilm in treatment facilities. This strain identified as Alcaligenes sp. through morphological, physiological biochemical examinations. After cultivation, enhanced by 26.3% its degradation capacity for 2,6-DTBP. Results indicated that able to utilize 2,6-DTBP, lysine, lactamine, citrate, n-utenedioic acid malic sole carbon energy source, alkalinize acetamide, asparagine, L-histidine, acetate, citrate propionate, but failed glucose, D-fructose, D-seminose, D-xylose, serine phenylalanine source. The optimal growth conditions were determined be: temperature 37 degrees C, pH 7.0, inoculum size 0.1% shaker rotary speed 250 r/min. Under conditions, kinetics 2,6-DTBP with initial concentration 100 mg/L studied. 62.4% removed after 11 d. could be expressed Eckenfelder equation a half life 9.38 In addition, played important role on ability strain. maximum 200 mg/L. Above this level, overloaded exhibited significant inhibition.