African freshwater eel species (Anguilla spp.) identification through DNA barcoding.

摘要: Freshwater eels (Anguilla spp.) have a long and complex catadromous life cycle. This unique feature, coupled with difficulty in separating species based on morphology, makes them targets for conservation. In this study we evaluated the utility of DNA barcoding using cytochrome oxidase I (COI) to delimit four African found western Indian Ocean region. We collected 76 individual fin clips from eel (A. mossambica, n=51; A. marmorata, n=17; bengalensis, n=6; bicolor, n=2) rivers KwaZulu–Natal, South Africa, 2016–18. Phylogenetic analysis COI sequences recovered all as monophyletic. Barcoding gap analyses were performed there was no overlap inter- intraspecific genetic distances. Consequently, use an identification tool be reliable identifying level, which suggests that marker should included future environmental or metabarcoding studies.