Development of a novel DNA-launched dengue virus type 2 infectious clone assembled in a bacterial artificial chromosome.

摘要: Major progress in Dengue virus (DENV) biology has resulted from the use of infectious clones obtained through reverse genetics. The construction these is commonly based on high- or low-copy number plasmids, yeast artificial chromosomes, yeast-Escherichia coli shuttle vectors, and bacterial chromosomes (BACs). Prokaryotic promoters have consistently been used for transcription clones. goal this study was to develop a novel DENV clone BAC under control cytomegalovirus immediate-early promoter generate with fusion envelope-green fluorescent protein an attempt track infection. transfection Vero cells plasmid encoding facilitated recovery particles that increased titer after serial passages C6/36 cells. plaque size syncytia phenotypes recombinant were similar those parental virus. Despite observation autonomous replication detection low levels viral genome two passages, insertion green Renilla luciferase reporter genes negatively impacted rescue. To best our knowledge, first using facilitate viruses without need vitro transcription. This molecular will be useful establishing basis replication, assembly, pathogenesis, evaluating potential antiviral drugs, development vaccine candidates attenuated viruses.