Early-appearing tumour-infiltrating natural killer cells play a crucial role in the generation of anti-tumour T lymphocytes.

摘要: Natural killer (NK) cells that infiltrated into the primary tumour site at an early stage of development, were examined for their participation in generation anti-tumour cytotoxic T lymphocytes (CTL). NK cells, which detected by anti-NK1.1 monoclonal antibody (mAb), increased peritoneal exudate (PEC) on days 3 and 7 after intraperitoneal (i.p.) inoculation syngeneic B16 melanoma cells. These tumour-infiltrating showed a high level activity against NK-sensitive YAC-1 expression interferon-gamma (IFN-gamma) mRNA interleukin-2 (IL-2) mRNA. The vivo depletion with mAb, prior to i.p. resulted number PEC compared cell non-depleted mice. Interestingly, differences between both groups more prominent 14 than day 3, strongly suggested early-infiltrating have large influence subsequent response. immunization abrogated capacity spleen generate CD8+ tumour-specific CTL vitro restimulation. This inability generating was partially restored additional injections recombinant IL-2 and/or IFN-gamma simultaneously restimulation impaired from immunized Lastly, abolished protective immunity rechallenge. Overall, these results indicate early-appearing not only participate defence themselves, but also play crucial role CTL.