A Mouse Model of Hepatic Ischemia-Reperfusion Injury Demonstrates Potentially Reversible Effects on Hippocampal Neurons and Postoperative Cognitive Function.

摘要: BACKGROUND This study aimed to investigate cognitive function, hippocampal neuronal changes, and the expression of inflammatory cytokines in a mouse model hepatic ischemia-reperfusion injury. MATERIAL AND METHODS Sixty mice were divided into sham group, which underwent surgery without vascular occlusion; I/R1 with occlusion left artery portal vein for 20 min, reperfusion 30 min; I/R2 40 min. At postoperative day 4 11, ten from each group Morris water maze (MWM) task. Hippocampal tissues stained Nissl bodies. Expression nuclear factor-κB (NF-κB) choline acetyltransferase (ChAT) quantified by immunohistochemistry. Serum tumor necrosis factor-α (TNF-α) interleukin-1β (IL-1β) measured enzyme-linked immunosorbent assay (ELISA). RESULTS Groups showed significantly increased latency MWM test between days 5-9, compared (P<0.05), no difference 11; had an initial lower crossing frequency 18. The reduced numbers bodies neurons. groups NF-κB, TNF-α, IL-1β decreased ChAT. No differences found levels IL-1β, or ChAT CONCLUSIONS A injury transient reversible dysfunction, changes neurons, cytokines.