Development of a new bimodal imaging methodology: a combination of fluorescence microscopy and high-resolution secondary ion mass spectrometry.

摘要: Summary In this paper, we present a new experimental methodology to combine mass spectrometry (NanoSIMS) with fluorescence microscopy provide subcellular information on the location of small molecules in cultured cells. We demonstrate by comparing distribution 5-bromo-2-deoxyuridine same cells given both NanoSIMS analysis and immunohistochemistry. Fiducial markers substrates ensured that images formed SIMS mapping bromine ions could be co-registered exactly from microscopy. The was shown faithfully reproduce microscopy, but at much higher spatial resolution. then show preliminary ATN-224, therapeutic copper chelator for which there is no fluorescent marker, conventional Lysotracker Hoechst stains