Assembly of recombinant TFIID reveals differential coactivator requirements for distinct transcriptional activators.
作者： Jin-Long Chen , Laura Donatella Attardi , C.Peter Verrijzer , Kyoko Yokomori , Robert Tjian
DOI: 10.1016/0092-8674(94)90403-0
关键词:
摘要: Abstract We previously reported that transcriptional regulators can bind selected TAF subunits of the TFIID complex. However, specificity and function individual TAFs in mediating activation remained unknown. Here we report vitro assembly properties TBF-TAF complexes reconstituted from nine recombinant Drosophila TFIID. A minimal complex containing TBP II 250 directs basal but not activator-responsive transcription. By contrast, holo-TFIID supports by an assortment activators. The activator NTF-1, which binds 150, stimulates transcription with a only TBP, 250, whereas Sp1 additionally requires 110 for activation. Interestingly, 150 enhances even though this subunit does directly to Sp1. These results establish specific subcomplexes mediate different classes activators suggest perform multiple functions during
elsevier.com
sci-hub.se 参考文章(37)
C. Verrijzer, K Yokomori, J. Chen, R Tjian, Drosophila TAFII150: similarity to yeast gene TSM-1 and specific binding to core promoter DNA. Science. ,vol. 264, pp. 933- 941 ,(1994) , 10.1126/SCIENCE.8178153
G. Gill, E. Pascal, Z. H. Tseng, R. Tjian, A glutamine-rich hydrophobic patch in transcription factor Sp1 contacts the dTAFII110 component of the Drosophila TFIID complex and mediates transcriptional activation Proceedings of the National Academy of Sciences of the United States of America. ,vol. 91, pp. 192- 196 ,(1994) , 10.1073/PNAS.91.1.192
Koji Hisatake, Satoshi Hasegawa, Ritsuko Takada, Yoshihiro Nakatanl, Masami Horikoshi, Robert G. Roeder, The p250 subunit of native TATA box-binding factor TFIID is the cell-cycle regulatory protein CCG1. Nature. ,vol. 362, pp. 179- 181 ,(1993) , 10.1038/362179A0
B.Franklin Pugh, Robert Tjian, Mechanism of transcriptional activation by Sp1: evidence for coactivators. Cell. ,vol. 61, pp. 1187- 1197 ,(1990) , 10.1016/0092-8674(90)90683-6
Mark Ptashne, How eukaryotic transcriptional activators work Nature. ,vol. 335, pp. 683- 689 ,(1988) , 10.1038/335683A0
T. Kokubo, D. W. Gong, R. G. Roeder, M. Horikoshi, Y. Nakatani, The Drosophila 110-kDa transcription factor TFIID subunit directly interacts with the N-terminal region of the 230-kDa subunit Proceedings of the National Academy of Sciences of the United States of America. ,vol. 90, pp. 5896- 5900 ,(1993) , 10.1073/PNAS.90.13.5896
Michael Meisterernst, Ananda L. Roy, Hsiao Mei Lieu, Robert G. Roeder, Activation of class II gene transcription by regulatory factors is potentiated by a novel activity Cell. ,vol. 66, pp. 981- 993 ,(1991) , 10.1016/0092-8674(91)90443-3
N Hernandez, TBP, a universal eukaryotic transcription factor Genes & Development. ,vol. 7, pp. 1291- 1308 ,(1993) , 10.1101/GAD.7.7B.1291
James A. Goodrich, Timothy Hoey, Catherine J. Thut, Arie Admon, Robert Tjian, Drosophila TAFII40 interacts with both a VP16 activation domain and the basal transcription factor TFIIB Cell. ,vol. 75, pp. 519- 530 ,(1993) , 10.1016/0092-8674(93)90386-5
Bob Choy, Michael R. Green, Eukaryotic activators function during multiple steps of preinitiation complex assembly. Nature. ,vol. 366, pp. 531- 536 ,(1993) , 10.1038/366531A0