FOXM1 confers to epithelial-mesenchymal transition, stemness and chemoresistance in epithelial ovarian carcinoma cells.

摘要: // Wen-Tai Chiu 1,2 , Yu-Fang Huang 3 Huei-Yu Tsai 4 Chien-Chin Chen 5,6 Chang-Hwa Chang 2 Soon-Cen 7 Keng-Fu Hsu 3,4 and Cheng-Yang Chou 1 Department of Biomedical Engineering, National Cheng Kung University, Tainan, Taiwan Institute Basic Medical Sciences, Obstetrics Gynecology, University Hospital, College Medicine, Cancer Research Center, 5 Pathology, Chia-Yi Christian Chiayi, 6 Cosmetic Science, Chia Nan Pharmacy Chi Mei Liouying Campus, Correspondence: Chou, email: Keywords : ovarian cancer, FOXM1, β-CATENIN, chemoresistance, stemness Received October 15, 2014 Accepted December 09, Published 10, Abstract Chemoresistance to anti-cancer drugs substantially reduces survival in epithelial cancer. In this study, we showed that chemoresistance cisplatin paclitaxel induced the epithelial-mesenchymal transition (EMT) a stem cell phenotype cancer cells. was associated with downregulation markers upregulation mesenchymal markers, EMT-related transcription factors, which enhanced invasion sphere formation ability. Overexpression FOXM1 increased cisplatin-resistance cisplatin-sensitive low FOXM1-expressing Conversely, depletion via RNA interference reduced resistance cisplatin-resistant high also expression, nuclear accumulation, activity β-CATENIN chemoresistant cells, whereas suppressed these events. The combination inhibitor thiostrepton inhibited expression cells subcutaneous tumor growth mouse xenografts. an analysis 106 patients, levels tumors were progression short progression-free intervals. Collectively, our findings highlight importance suggest inhibitors may be useful for treatment